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81.
[目的]在基础日粮的水平上添加中药复方,纯中药全程饲喂,对保育阶段仔猪血清中胆固醇和脂蛋白含量进行测定,为该中药复方对保育阶段仔猪增强机体抵抗力,降低抗生素在畜牧业的应用,形成质量可靠的“无抗”猪肉提供帮助。[方法]试验选用80头(28±2)日龄、体重相近的“杜×长×大”三元杂交断奶仔猪,随机分为4个处理,每个处理2个重复,每个重复10头仔猪。第1组为对照组,饲喂基础日粮,第2、3、4组在饲喂基础日粮的基础上分别添加1.5%、1.0%和0.5%的中药复方,试验期4周。试验结束第2天上午9:00空腹,前腔静脉采血,进行全血血常规指标测定。[结果]与对照组相比,饲料中添加中药复方组保育仔猪血清中脂蛋白脂肪酶含量均有升高,其中,添加0.5%中药复方组与对照组相比差异显著(P<0.05);与对照组相比,饲料中添加中药复方组保育仔猪血清中高密度脂蛋白含量均有升高,其含量随中药复方添加量的减少而逐渐降低,各中药复方添加组与对照组相比差异均不显著(P>0.05);与对照组相比,饲料中添加中药复方组保育仔猪血清中低密度脂蛋白含量均有所下降,其含量随中药复方添加量的减少而逐渐降低,其中,添加0.5%中药复方组与对照组相比差异极显著(P<0.01);与对照组相比,饲料中添加中药复方组保育仔猪血清中三酰甘油含量均有下降,其含量随中药复方添加量的减少而逐渐降低,各中药复方添加组与对照组相比差异均不显著(P>0.05)。[结论]在基础日粮中添加中药复方组保育仔猪血清中脂蛋白脂肪酶含量、高密度脂蛋白含量均有升高;保育仔猪血清中低密度脂蛋白含量、三酰甘油含量均有降低。 相似文献
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Peirong Li Tongbing Su Huiping Wang Xiuyun Zhao Weihong Wang Yangjun Yu Deshuang Zhang Changlong Wen Shuancang Yu Fenglan Zhang 《Plant Breeding》2019,138(3):309-324
Single‐nucleotide polymorphisms (SNPs) are rapid, economical and reliable genotyping tools. Non‐heading Chinese cabbage (Brassica rapa L. subsp. chinensis Makino) is now an economically important vegetable crop worldwide. In this study, 1,167 SNPs were evaluated for 7polymorphism among 70 representative non‐heading Chinese cabbage inbred lines using a Kompetitive Allele Specific PCR (KASP) genotyping assay. On the basis of identified polymorphisms and the results of a principal component analysis, we selected 50 core SNPs that were balanced sufficiently to provide adequate information for genetic identification. The core SNPs were used for construction of a neighbour‐joining dendrogram that separated the 70 inbred lines into four main groups and several subgroups corresponding to Caixin, Heiyebaicai, Huangxinwu, Naibaicai, Taitsai, Pak‐choi, and Wutatsai. This categorization was superior to that achieved using a dataset of 479 polymorphic SNPs. To confirm the utility of the core SNP markers in genetic identification, we tested their stability and resolution using 162 commercial hybrid cultivars. The SNPs, which represent a cost‐effective, accurate marker set for germplasm analysis and cultivar identification, are suitable for molecular marker‐assisted breeding in non‐heading Chinese cabbage. 相似文献
85.
通过优化大黄药渣固体培养基的组成及发酵条件,以期最大程度提高发酵生产漆酶的活力。采用D-最优混料设计对固体培养基碳源组成进行优化,优化后得到的固体培养基组成为:小麦麸皮47.2%,大黄药渣47.2%,葡萄糖1.6%,蛋白胨2.5%,氯化钙0.48%,磷酸二氢钾0.5%,硫酸铜0.02%,硫酸镁0.5%。通过中心组合设计响应面试验建立二次回归模型对固态发酵条件进行优化,确定最优发酵条件为发酵初始pH 4.3,发酵温度32.5℃,固液质量体积比1.47∶1,发酵时间7.5 d。在此条件下,发酵生产的漆酶酶活为7 602.1 IU/mL,与模型预测值相对误差在0.6%。 相似文献
86.
对于投资国来说,对外直接投资的产业选择对投资收益和潜在收益有较大影响。为了研究中国对美直接投资的产业选择对中国产业的影响,本文基于比较优势,构建发展中国家OFDI决策模型,通过构造评价指标以及构建回归模型,并利用2005-2014年中国对外直接投资数据、出口数据、行业相关数据对中国对美直接投资的效应进行了实证分析。结果表明,作为中国对美直接投资的两个重点产业,第二、第三产业可以为提高本国对应产业的比较优势提供支撑;中国对美直接投资、美国自身的产业比较优势、其他国家对中国直接投资、中国国内的固定资产投资、中国各行业从业人员数均有助于提高中国产业比较优势;由于中国内部约束和外部约束的原因,中国对美直接投资对提升中国产业比较优势的作用还不明显。 相似文献
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The objective of this study was to evaluate of the growth performance, serum biochemical indices, liver antioxidant parameters and ability of anti-infection with Aeromonas hydrophila by adding the fermented Chinese medicine by Bacillus Subtilis to the carp fish feed, and to find out the appropriate supplemental level of fermented Chinese medicine in diets. Carp fingerling with an initial body weight of (35±0.5) g were set up to 6 experimental diets supplemented with 0 (control group), 0.1‰, 0.5‰, 1‰, 2‰ and 3‰ fermented Chinese medicine for 56 days, respectively. Each group had 30 fish and set up 3 repetitions. After the feeding trail,the fish were challenged with Aeromonas hydrophila to calculate the relative percentage survival (RPS).The results showed as follows:Supplementing fermented traditional Chinese medicine by Bacillus subtilis, the weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), the activities of lysozyme (LSZ), complement 3 (C3),total protein (TP),total anti-oxidation capacity (T-AOC),total superoxide dismutase (T-SOD) and catalase (CAT) in serum of common carp were enhanced at different degrees, while the feed coefficient (FCR)and malondialdehyde (MDA) in serum were declined at different degrees.When the fermented Chinese medicine supplemental levels were 2‰ and 3‰,the effects were significant (P<0.05)(except for C3, ALP and Cr).The experimental results of the virulence of common carp infected with pathogen Aeromonas hydrophila showed that,the fermented herbal could obviously improve the ability of anti-infection, the immune protective rate of the group with 2‰, 3‰ doses of the fermented Chinese medicine could reach 77.23% and 74.63%, respectively. From what had been discussed above, the group with 2‰ and 3‰ doses of the fermented Chinese medicine had significant effect on the growth performance, serum biochemical indices, liver antioxidant parameters and Aeromonas hydrophila anti-infection ability. Considering the cost of adding fermented Chinese medicine, the recommendations for the best additive quantity was 2‰. 相似文献
89.
The paper is a brief introduction of porcine endogenous retrovirus (PERV) innovative research results in the world and also expounds the passing PERV existence situation on different varieties of miniature pig, analyzes the PERV-virus gene cloning and sequence, appraises method on cell level, create the platform of infection HEK293 cells research, study on pigs A3F inhibition of PERV, and reveal the innovative research results on the specific molecular genetics in the different strain of PERV, analyzes the advantages of Wuzhishan miniature pig inbred line such as low gene copy of PERV,and there is no passing PERV-C specificity and as well as looking forward to cultivate the methods for new strain of PERV negative pigs. It will provide a scientific counter measure and new perspective to solve the spread of disease risk of miniature pig PERV and product safety for human xenotransplantation and biomedical materials of research and development. 相似文献
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